Beamlines SER-CAT operates two state-of-the-art Insertion Device beamlines at Sector 22 of the Advanced Photon Source. These facilities provide advanced macromolecular crystallography capabilities for structural biology research, featuring complementary wavelength capabilities and automated sample handling systems. Beamline 22-ID-D: Variable Wavelength Operations 22-ID-D Technical Specifications X-ray Detection System: Primary Detector: Dectris Eiger X16M Fluorescence Detection: Amp-Tek 123 SDD X-ray Spectrometer Minimum Crystal-to-Detector Distance: 150 mm Energy and Wavelength Capabilities: Energy Range: 6.0 - 24.0 keV Wavelength Range: 2.1 - 0.52 Ångström (approximate) Configuration: Variable wavelength operations for multi-wavelength anomalous diffraction (MAD) experiments Goniometer and Sample Handling: Diffractometer: MD3 vertical omega-axis system (Arinax) Sample Mounting: Automated crystal mounting system compatible with MD3 platform Sample Capacity: 20 Unipuck positions with complete pin accessibility Additional 10 Unipuck positions with partial accessibility (pins 6, 7, 16 restricted) Beam Characteristics: Flux: 7 × 10¹² photons/second Beam Size: 10 μm × 10 μm (FWHM) Control Software: SERGUI beamline control and data collection platform Beamline 22-ID-E: High-Throughput Fixed Wavelength 22-ID-E Technical Specifications X-ray Detection System: Primary Detector: Rayonix MX300-HS high-speed detector Fluorescence Detection: Amp-Tek FAST 3DD XR-100 SDD X-ray Detector Minimum Crystal-to-Detector Distance: 150 mm Energy and Wavelength Capabilities: Fixed Energy: 12.700 keV Fixed Wavelength: 0.97 Ångström (approximate) Configuration: Optimized for high-throughput native data collection Goniometer and Sample Handling: Diffractometer: MD2 horizontal omega-axis system (Arinax) Sample Mounting: Automated crystal mounting system compatible with MD2 platform Sample Capacity: 6 Unipuck positions with complete pin accessibility Mounting Efficiency: 30-second dismount/mount cycle time Beam Characteristics: Flux: 7 × 10¹² photons/second Beam Size: 10 μm × 10 μm (FWHM) Control Software: SERGUI beamline control and data collection platform Comparative Beamline Parameters Specification 22-ID-D 22-ID-E Primary Application Variable wavelength MAD/SAD High-throughput screening Energy Range 6,000 - 24,000 eV 12,700 eV (fixed) Detector Technology Dectris Eiger X16M Rayonix MX300-HS Beam Flux 7 × 10¹² ph/s 7 × 10¹² ph/s Beam Size (FWHM) 10 μm × 10 μm 10 μm × 10 μm Minimum Detector Distance 150 mm 150 mm Sample Capacity (full access) 20 Unipuck positions 6 Unipuck positions Sample Exchange Time TBD 30 seconds Diffractometer Orientation Vertical omega-axis Horizontal omega-axis Research Capabilities and Applications 22-ID-D Applications Multi-wavelength Anomalous Diffraction (MAD): Comprehensive phasing experiments Single-wavelength Anomalous Diffraction (SAD): De novo structure determination High-resolution Data Collection: Detailed structural analysis projects Large Unit Cell Systems: Extended sample-to-detector distances available Specialized Energy Requirements: Optimized absorption edge studies 22-ID-E Applications High-throughput Screening: Rapid crystal evaluation and optimization Native Data Collection: Routine structural determinations Serial Crystallography: Automated sample processing workflows Time-sensitive Projects: Rapid data collection protocols Large-scale Structural Programs: Efficient sample throughput Advanced Features and Automation Data Collection Software SERGUI Platform: Integrated beamline control and data collection interface Automated data processing and evaluation pipelines Remote access capabilities for off-site users Real-time data quality assessment and feedback Sample Handling Systems Automated Crystal Mounting: Robotic sample handling with precise positioning accuracy Climate-controlled sample storage and transfer Integrated sample tracking and identification systems Emergency sample recovery and storage protocols
