Data Collection at SER-CAT

Learn some information that you need to collect high-quality crystallography data at SER-CAT. 

This page covers the SERGUI data organization protocols, wavelength configuration, automated sample mounting systems, and complete collection procedures for native and MAD experiments. Access step-by-step guides, alignment tools, and troubleshooting resources for successful remote or on-site data collection.


Data Collection Features & Tools

Select a topic below to learn more about each data collection feature:

Data Collection Protocols

Choose from default naming, Excel spreadsheet imports, or pre-defined templates to organize your data and prevent accidental file overwrites.

Energy & Wavelength Configuration

Configure wavelength settings for variable (22-ID-D: 6-24 keV) or fixed (22-ID-E: 12.7 keV) energy experiments including MAD/SAD phasing.

Beamline Tools & Standards

Access robot-controlled YAG crystals, alignment pins, and trypsin standards for beam alignment and goniometer verification.

Automated Sample Mounting

Robotic systems provide automated Unipuck handling with 30-second mount cycles and capacity for 20-30 pucks on both beamlines.

Data Collection Procedures

Step-by-step workflows guide you through native data collection, MAD experiments, sample rastering, and helical scans.


Frequently Asked Questions (FAQ)

A certain number of questions tend to arise frequently; you might be able to find your answer below:

  • Useful Reminders are included in this pdf, including answers to:
    • I started a data collection and I see blank images with no diffraction
    • APS stored beam was lost and it just came back; what should I do?
    • I can not see my sample
    • I am not able to open the "sergui" program
    • I collected some data sets and I would like to quickly look at auto-processing results
    • Where can I find the beam center values?
    • I sent too many samples and have lost track of which crystals I have already mounted; do you have that information somewhere?
    • My NX-client fails to connect to the SER-CAT computers